MBD1 Antikörper (C-Term)

Produktnummer ABIN2779420

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MBD1 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Maus und Kaninchen und wurde in 1 Publikation erwähnt.

Kurzübersicht für MBD1 Antikörper (C-Term) (ABIN2779420)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reaktivität: Human, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Maus, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MBD1 Antikörper

Bindungsspezifität: C-Term

Sequenz: NKDDSASKLA PEEEAGGAGT PVITEIFSLG GTRFRDTAVW LPRSKDLKKP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MBD1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MBD1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 549 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MBD1 Antikörper (ABIN2779420)

Zhao, Wu, Bu, Lu, Liang, Cao, Tong, Lu, Wu, Guo et al.: "Epigenetic silence of ankyrin-repeat-containing, SH3-domain-containing, and proline-rich-region- containing protein 1 (ASPP1) and ASPP2 genes promotes tumor growth in hepatitis B virus-positive ..." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 1, pp. 142-53, (2009) (PubMed).

Details zu MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Andere Bezeichnung: MBD1

Hintergrund: MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
Alias Symbols: CXXC3, PCM1, RFT
Protein Interaction Partner: SUMO1, HTT, UBC, ECT2, APP, ATXN1L, TENM1, SUMO2, HIST1H3A, PCNA, ATF7IP2, ATF7IP, CHAF1A, SETDB1, PIAS1, STAT1, PRAM1, HDAC3, PIAS2, ZNF512B, Rnf2, CBX4, CBX5, HIPK3, SMAD9, OASL, SMAD5, SMAD3, SMAD7, SMAD1, SUV39H1,
Protein Size: 549

Molekulargewicht: 60 kDa

Gen-ID: 4152

NCBI Accession: NM_015844, NP_056669