DFFA Antikörper (N-Term)

Produktnummer ABIN2779329

Der Kaninchen Polyklonal anti-DFFA Antikörper (ABIN2779329) detektiert spezifisch DFFA in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen und Pferd.

Kurzübersicht für DFFA Antikörper (N-Term) (ABIN2779329)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DFFA Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: DFFA Antibody - N-terminal region

Sequenz: MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL

Homologie: Cow: 92%, Dog: 83%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 83%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against DFFA. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099047

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Andere Bezeichnung: DFFA

Hintergrund:

Targeted Subunit: alpha

Background Information: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: DNA fragmentation factor, 45 kDa, alpha polypeptide

Alternative Symbols: DFF1, ICAD, DFF-45

Protein Name: DNA fragmentation factor subunit alpha

Molekulargewicht: 37kDa

Gen-ID: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Kaskade in der Apoptose