GAPDH Antikörper (N-Term)

Produktnummer ABIN2778645

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GAPDH in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Kaninchen, Hund, Schaf, Rind (Kuh), Pferd, Meerschweinchen und Ziege und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für GAPDH Antikörper (N-Term) (ABIN2778645)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Kaninchen, Hund, Schaf, Rind (Kuh), Pferd, Meerschweinchen, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: N-Term

Sequenz: IDLNYMVYMF QYDSTHGKFH GTVKAENGKL VINGNPITIF QERDPSKIKW

Homologie: Cow: 93%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against GAPDH. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 335 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-GAPDH Antikörper (ABIN2778645)

Kalinec, Thein, Parsa, Yorgason, Luxford, Urrutia, Kalinec: "Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways." in: Hearing research, Vol. 313, pp. 26-37, (2014) (PubMed).

Fu, Mukhamedova, Ip, DSouza, Henley, DiTommaso, Kesani, Ditiatkovski, Jones, Lane, Jennings, Smyth, Kile, Sviridov: "ABCA12 regulates ABCA1-dependent cholesterol efflux from macrophages and the development of atherosclerosis." in: Cell metabolism, Vol. 18, Issue 2, pp. 225-38, (2013) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: G3PD, GAPD, MGC88685
Protein Interaction Partner: FUS, HUWE1, UBC, SUMO2, SUMO3, STAU1, MDM2, GAPDH, ASB15, ASB16, RPA3, RPA2, RPA1, EED, SNCA, rev, HSPA9, HSPA1L, GAS7, TUBA1A, TUFM, RAP1GDS1, PPP5C, PPIB, YWHAG, TUBB, TUBA1C, PRPF40A, UBQLN1, DCPS, MAT2B, ACOT7, METAP2, FERMT2, GBP1, EEF1A1, TYMP, DFFA
Protein Size: 335

Molekulargewicht: 36 kDa

Gen-ID: 2597

NCBI Accession: NM_002046, NP_002037

UniProt: Q2TSD0