Nanog Antikörper (Middle Region)

Produktnummer ABIN2777175

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Nanog in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Schaf, Ziege, Pferd, Kaninchen und Meerschweinchen und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Nanog Antikörper (Middle Region) (ABIN2777175)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schaf, Ziege, Pferd, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: Middle Region

Sequenz: SYKQVKTWFQ NQRMKSKRWQ KNNWPKNSNG VTQKASAPTY PSLYSSYHQG

Homologie: Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 86%, Rat: 92%, Sheep: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NANOG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 305 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-Nanog Antikörper (ABIN2777175)

Kallas, Pook, Maimets, Zimmermann, Maimets: "Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells." in: PLoS ONE, Vol. 6, Issue 4, pp. e19114, (2011) (PubMed).

Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, (2010) (PubMed).

Neganova, Zhang, Atkinson, Lako: "Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells." in: Oncogene, Vol. 28, Issue 1, pp. 20-30, (2009) (PubMed).

Maimets, Neganova, Armstrong, Lako: "Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells." in: Oncogene, Vol. 27, Issue 40, pp. 5277-87, (2008) (PubMed).

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG

Hintergrund: NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305

Molekulargewicht: 34 kDa

Gen-ID: 79923

NCBI Accession: NM_024865, NP_079141

UniProt: Q6NSW7

Pathways: Stem Cell Maintenance