ube3a Antikörper (N-Term)

Produktnummer ABIN2775647

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ube3a in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Kaninchen, Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Rind (Kuh), Saccharomyces cerevisiae und Ziege.

Kurzübersicht für ube3a Antikörper (N-Term) (ABIN2775647)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reaktivität: Human, Maus, Ratte, Kaninchen, Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Rind (Kuh), Saccharomyces cerevisiae, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ube3a Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: UBE3A Antibody - N-terminal region

Sequenz: SLQAKDEDKD EDEKEKAACS AAAMEEDSEA SSSRIGDSSQ GDNNLQKLGP

Homologie: Cow: 93%, Dog: 93%, Goat: 90%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Yeast: 91%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against UBE3A. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE3A

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42950-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A

Hintergrund:

Background Information: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Gene Name: Ubiquitin protein ligase E3A

Alternative Symbols: AS, ANCR, E6-AP, HPVE6A, EPVE6AP

Protein Name: Ubiquitin-protein ligase E3A

Molekulargewicht: 101kDa

Gen-ID: 7337

NCBI Accession: NP_000453

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway