GNAS Antikörper (N-Term)

Produktnummer ABIN2775542

Der Kaninchen Polyklonal Anti-GNAS-Antikörper wurde für WB und IHC validiert. Er ist geeignet, GNAS in Proben von Human, Maus, Ratte, Hund, Rind (Kuh), Kaninchen, Pferd und Meerschweinchen zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für GNAS Antikörper (N-Term) (ABIN2775542)

Target: GNAS (GNAS Complex Locus (GNAS))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Kaninchen, Pferd, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GNAS Antikörper

Bindungsspezifität: N-Term

Sequenz: VYRATHRLLL LGAGESGKST IVKQMRILHV NGFNGEGGEE DPQAARSNSD

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against GNAS. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GNAS

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 394 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-GNAS Antikörper (ABIN2775542)

Moreno-Smith, Lee, Lu, Nagaraja, He, Rupaimoole, Han, Jennings, Roh, Nishimura, Kang, Allen, Armaiz, Matsuo, Shahzad, Bottsford-Miller, Langley, Cole, Lutgendorf, Siddik, Sood: "Biologic effects of dopamine on tumor vasculature in ovarian carcinoma." in: Neoplasia (New York, N.Y.), Vol. 15, Issue 5, pp. 502-10, (2013) (PubMed).

Masyuk, Huang, Radtke, Gajdos, Splinter, Masyuk, Gradilone, LaRusso: "Ciliary subcellular localization of TGR5 determines the cholangiocyte functional response to bile acid signaling." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 304, Issue 11, pp. G1013-24, (2013) (PubMed).

Details zu GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Andere Bezeichnung: GNAS

Hintergrund: Mutations in GNAS gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Alias Symbols: AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, NESP, PHP1C
Protein Interaction Partner: PANX1, AXIN1, UBC, FUS, OPTN, PTGIR, HLA-A, ADRB2, NUCB2, NUCB1, LAMTOR1, SLC25A12, GNAQ, GNA11, UBD, TBXA2R, GNB1, AVPR2, SUMO1, PCK1, Ric8b, GNG2, CALM1, Haus1, Trim69, Cbx1, RIC8A, TTC1, SNX13, ADCY5, CRHR1, PTGDR, TSHR, CAV3, HTR6, RGS2, ADCY6, VIPR1,
Protein Size: 394

Molekulargewicht: 46 kDa

Gen-ID: 2778

NCBI Accession: NM_000516, NP_000507

UniProt: P63092

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis