MCM4 Antikörper (N-Term)

Produktnummer ABIN2774893

Der Kaninchen Polyklonal anti-MCM4 Antikörper wird verwendet zum Nachweis von MCM4 in Proben von Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Hund und Pferd. Er wurde validiert für WB.

Kurzübersicht für MCM4 Antikörper (N-Term) (ABIN2774893)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Hund, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: MCM4 Antibody - N-terminal region

Sequenz: SRVEGTPRSG VRGTPVRQRP DLGSAQKGLQ VDLQSDGAAA EDIVASEQSL

Homologie: Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM4

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36662-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund:

Background Information: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Gene Name: Minichromosome maintenance complex component 4

Alternative Symbols: NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21

Protein Name: DNA replication licensing factor MCM4

Molekulargewicht: 95kDa

Gen-ID: 4173

NCBI Accession: NP_877423

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA