MCM4 Antikörper (Middle Region)

Produktnummer ABIN2774884

Produktdetails anti-MCM4 Antikörper

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Hund, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: VYKTHIDVIH YRKTDAKRLH GLDEEAEQKL FSEKRVELLK ELSRKPDIYE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MCM4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 863 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4 (MCM4 Produkte)

Synonyme: cdc21 antikoerper, CDC21 antikoerper, CDC54 antikoerper, NKCD antikoerper, NKGCD antikoerper, P1-CDC21 antikoerper, hCdc21 antikoerper, 19G antikoerper, AI325074 antikoerper, AU045576 antikoerper, Cdc21 antikoerper, Mcmd4 antikoerper, mKIAA4003 antikoerper, mcdc21 antikoerper, cb1025 antikoerper, fc12c09 antikoerper, fj85g09 antikoerper, hm:zeh1616 antikoerper, wu:fc12c09 antikoerper, wu:fj85g09 antikoerper, zeh1616 antikoerper, minichromosome maintenance complex component 4 L homeolog antikoerper, minichromosome maintenance complex component 4 S homeolog antikoerper, minichromosome maintenance complex component 4 antikoerper, mcm4.L antikoerper, mcm4.S antikoerper, MCM4 antikoerper, mcm4 antikoerper, Mcm4 antikoerper

Hintergrund: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
Alias Symbols: CDC21, CDC54, hCdc21, P1-CDC21
Protein Interaction Partner: HUWE1, UBC, PCNA, SUMO2, FKBP5, MCMBP, MDM2, POLK, CLUH, NCKAP1, CAP1, CAP2, EIF3B, MCM2, KPNA3, PRMT1, FKBP4, MCM7, MCM6, FBXW4, FBXO46, ITGA4, CDC5L, gag-pol, ORC6, CCNA1, VCAM1, DBF4, CDC7, LIG4, MCM5, MCM3, DCTN2, SF3A1, UBD, NEDD8, TONSL, SIRT7, ASF1
Protein Size: 863

Molekulargewicht: 95 kDa

Gen-ID: 4173

NCBI Accession: NM_005914, NP_005905

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA