MCM4 Antikörper (Middle Region)

Produktnummer ABIN2774884

Dieses Anti-MCM4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MCM4 in WB und IHC. Geeignet für Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Hund, Pferd und Zebrafisch (Danio rerio).

Kurzübersicht für MCM4 Antikörper (Middle Region) (ABIN2774884)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Hund, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: Middle Region

Sequenz: VYKTHIDVIH YRKTDAKRLH GLDEEAEQKL FSEKRVELLK ELSRKPDIYE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MCM4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 863 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
Alias Symbols: CDC21, CDC54, hCdc21, P1-CDC21
Protein Interaction Partner: HUWE1, UBC, PCNA, SUMO2, FKBP5, MCMBP, MDM2, POLK, CLUH, NCKAP1, CAP1, CAP2, EIF3B, MCM2, KPNA3, PRMT1, FKBP4, MCM7, MCM6, FBXW4, FBXO46, ITGA4, CDC5L, gag-pol, ORC6, CCNA1, VCAM1, DBF4, CDC7, LIG4, MCM5, MCM3, DCTN2, SF3A1, UBD, NEDD8, TONSL, SIRT7, ASF1
Protein Size: 863

Molekulargewicht: 95 kDa

Gen-ID: 4173

NCBI Accession: NM_005914, NP_005905

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA