MCM2 Antikörper (N-Term)

Produktnummer ABIN2774882

Der Kaninchen Polyklonal Anti-MCM2-Antikörper wurde für WB validiert. Er ist geeignet, MCM2 in Proben von Human, Maus, Ratte, Hund, Pferd, Kaninchen, Rind (Kuh), Meerschweinchen und Schwein zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für MCM2 Antikörper (N-Term) (ABIN2774882)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Rind (Kuh), Meerschweinchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MCM2 Antikörper

Bindungsspezifität: N-Term

Sequenz: PGRSSRRTDA LTSSPGRDLP PFEDESEGLL GTEGPLEEEE DGEELIGDGM

Homologie: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against MCM2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM2

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 904 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MCM2 Antikörper (ABIN2774882)

Rizwani, Alexandrow, Chellappan: "Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 10, pp. 1621-9, (2009) (PubMed).

Details zu MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Andere Bezeichnung: MCM2

Hintergrund: The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Alias Symbols: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN
Protein Interaction Partner: HUWE1, MCM6, CHFR, SUMO2, MCMBP, UBC, SUMO1, MDM2, MCM7, MCM4, HSP90AB1, ACLY, PRRC1, HYOU1, CAP1, NCL, POLA1, TONSL, SNF8, ELL, TCEA1, POLR2A, CDK6, CDK4, CDC6, MCM10, ORC6, CCNB2, VCAM1, P2RX4, ORC5, ORC2, MCM5, MCM3, ITGA4, FN1, CSNK2A1, CDKN2A, DBF4,
Protein Size: 904

Molekulargewicht: 99 kDa

Gen-ID: 4171

NCBI Accession: NM_004526, NP_004517

UniProt: P49736

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA