ERCC1 Antikörper

Produktnummer ABIN2763838

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ERCC1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für ERCC1 Antikörper (ABIN2763838)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERCC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ERCC1 Antikörper

Kreuzreaktivität: Human

Produktmerkmale: Excision Repair Cross Complementing protein-1, Nucleotide Excision Repair Protein,Recognizes a protein of 33-36 kDa, identified as the Excision Repair Cross Complementing (ERCC1). The mammalian ERCC1-encoded polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts, and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.

Aufreinigung: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide derived from human ERCC1 protein

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: This antibody can be used for Western blotting (5-10 μg/mL) and immunohistochemistry on formalin/paraffin sections (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1 (DNA excision repair protein)

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response