Ki-67 Antikörper (PE)

Produktnummer ABIN2749194

Der Maus Polyklonal Anti-Ki-67-Antikörper wurde für ICFC validiert. Er ist geeignet, Ki-67 in Proben von Human und Rind (Kuh) zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Ki-67 Antikörper (PE) (ABIN2749194)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Polyklonal

Konjugat: Dieser Ki-67 Antikörper ist konjugiert mit PE

Applikation: Intracellular Flow Cytometry (ICFC)

Produktdetails anti-Ki-67 Antikörper (PE)

Verwendungszweck: Anti-Ki-67 PE

Spezifität: The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Kreuzreaktivität (Details): Human, Bovine

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Nuclei of the Hodgkin lymphoma cell line L428

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Ki-67 Antikörper (PE) (ABIN2749194)

Schlüter, Duchrow, Wohlenberg, Becker, Key, Flad, Gerdes: "The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins." in: The Journal of cell biology, Vol. 123, Issue 3, pp. 513-22, (1993) (PubMed).

Gerdes, Lemke, Baisch, Wacker, Schwab, Stein: "Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1710-5, (1984) (PubMed).

Gerdes, Schwab, Lemke, Stein: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, (1983) (PubMed).

Details zu Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Andere Bezeichnung: Ki-67

Hintergrund: Marker of proliferation Ki-67,Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.,KIA, MIB, Marker of proliferation Ki67, MKI67

Gen-ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process