Dieser CD235a/GYPA Antikörper ist konjugiert mit PE
Applikation
Flow Cytometry (FACS)
Verwendungszweck
Anti-Hu CD235a PE
Spezifität
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Kreuzreaktivität (Details)
Human, Rat
Aufreinigung
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogen
Membrane preparation from splenic hairy cell leukemia
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Yamauchi, Takenaka, Urata, Shima, Kikushige, Tokuyama, Iwamoto, Nishihara, Iwasaki, Miyamoto, Honma, Nakao, Matozaki, Akashi: "Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment." in: Blood, Vol. 121, Issue 8, pp. 1316-25, (2013) (PubMed).
Beck, Jagodzinski, Eller, Thelian, Matyas, Kunz, Alving: "Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients." in: PLoS ONE, Vol. 8, Issue 11, pp. e81002, (2013) (PubMed).
Alijotas-Reig, Palacio-Garcia, Llurba, Vilardell-Tarres: "Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review." in: Fertility and sterility, Vol. 99, Issue 2, pp. 441-9, (2013) (PubMed).
Maijenburg, Kleijer, Vermeul, Mul, van Alphen, van der Schoot, Voermans: "The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging." in: Haematologica, Vol. 97, Issue 2, pp. 179-83, (2012) (PubMed).
Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group