CD8B Antikörper (FITC)

Produktnummer ABIN2749076

Produktdetails anti-CD8B Antikörper (FITC)

Target: CD8B (CD8b Molecule (CD8B))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8B Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Verwendungszweck: Anti-Rt CD8b FITC

Spezifität: The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

Kreuzreaktivität (Details): Rat

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: CD8 positive Wistar rat splenic T cell hybridomas

Klon: 341

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD8B Antikörper (FITC) (ABIN2749076)

Mabarrack, Turner, Mayrhofer: "Recent thymic origin, differentiation, and turnover of regulatory T cells." in: Journal of leukocyte biology, Vol. 84, Issue 5, pp. 1287-97, (2008) (PubMed).

Nohara, Kulka, Déry, Wills, Hirji, Gilchrist, Befus: "Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5935-9, (2001) (PubMed).

Ellerman, Like: "Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat." in: Diabetes, Vol. 48, Issue 5, pp. 975-82, (1999) (PubMed).

Lin, Hirji, Nohara, Stenton, Gilchrist, Befus: "Mast cells express novel CD8 molecules that selectively modulate mediator secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6265-72, (1998) (PubMed).

Hirji, Lin, Bissonnette, Belosevic, Befus: "Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6004-11, (1998) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).

Details zu CD8B

Target: CD8B (CD8b Molecule (CD8B))

Andere Bezeichnung: CD8b (CD8B Produkte)

Hintergrund: CD8b molecule,The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

Gen-ID: 926

UniProt: P10966

Pathways: T-Zell Rezeptor Signalweg