CD13 Antikörper

Produktnummer ABIN2749014

Der Maus Monoklonal Anti-CD13-Antikörper wurde für FACS, IHC (fro), IP und Func validiert. Er ist geeignet, CD13 in Proben von Human und Non-Human Primate zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD13 Antikörper (ABIN2749014)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD13 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Functional Studies (Func)

Klon: WM15

Produktdetails anti-CD13 Antikörper

Verwendungszweck: Anti-Hu CD13 Purified Low Endotoxin

Spezifität: The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human AML cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Functional application: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 Molecule immunoprecipitates.
Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD13 Antikörper (ABIN2749014)

McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, (2013) (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, (1993) (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Berndt, Musgrove: "Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells." in: Pathology, Vol. 17, Issue 3, pp. 392-9, (1986) (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Musgrove: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, (1985) (PubMed).

Details zu CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Andere Bezeichnung: CD13

Hintergrund: Alanyl aminopeptidase, membrane,CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.,Aminopeptidase N, APN, PEPN, ANPEP, gp150, LAP1

Gen-ID: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones