LAMP1 Antikörper (AA 300-350)

Produktnummer ABIN2746833

Der Kaninchen Polyklonal Anti-LAMP1-Antikörper wurde für WB, IP, ELISA und DB validiert. Er ist geeignet, LAMP1 in Proben von Human und Affe zu detektieren.

Kurzübersicht für LAMP1 Antikörper (AA 300-350) (ABIN2746833)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LAMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)

Produktdetails anti-LAMP1 Antikörper

Bindungsspezifität: AA 300-350

Spezifität: The antibody labels a strong band of LAMP1 at 50 kDa in ABIN1741625 samples and in other cancer cell lines.

Kreuzreaktivität: Affe

Kreuzreaktivität (Details): Does not cross react to any other cellular protein.

Produktmerkmale: LAMP1 selective antibodies were generated against a peptide taken from the human protein. The LAMP1-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The LAMP1- selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides PKCeta Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for LAMP1 antibody is also available, please inquire before ordering.

Aufreinigung: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 300-350 on human LAMP1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Synonyms: lampa, lgp120, CD107a.,LGP-120

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.55-0.75 μg/μL

Handhabung: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: LAMP1

Hintergrund: Lysosomes are membrane-bound vacuoles which play a critical role in endocytosis, synthesis and assembly of acid hydrolases. These sites are also site for digestion of foreign materials and for specialized autolytic cellular processes. Lysosome associated membrane proteins 1 and 2 (LAMP1/CD107a and LAMP2/CD107b) are members of the lysosome associated membrane glycoprotein family and are well known lysosomal markers. The disulfide arrangement is common to both members of this family of lysosomal membrane glycoproteins as observed by conservation of cysteine residues among lysosome-associated membrane proteins 1 and 2. LAMP1, also known as CD107a, is a heavily glycosylated membrane protein which has an important role in normal lysosomal function. This glycoprotein provides selectins with carbohydrate ligands and is a crucial mediator for cellular adhesion by interacting with carbohydrate receptors.It contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. LAMP1 plays a role in tumor cell invasion and metastasis. It is involved in lysosomal membrane protection and in lectin-mediated cell adhesion, and is also a marker of degranulation on lymphocytes such as CD8+ and NK cells. LAMP1, also called LAMPA, LGP120 or CD107a, is a member of a family of membrane glycoproteins and plays an important role in tumor cell metastasis. LAMP1 expression correlates with the metastatic potential of some tumor cells, and with neurons and glial cells surrounding plaques in Alzheimer's patients. LAMP1 also serve as a marker of mast cell activation and regulate the biogenesis of pigment melanin. LAMP1 is a 100-120 kDa type I integral lysosomal membrane glycoprotein that shuffles between lysosomes, phagosomes, and the plasma membranes. It is a transmembrane protein which is heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, protecting the proteins from proteolysis. The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes. LAMP1 and LAMP2 are 37 % homologous in their protein sequences and both are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking. LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and is involved in the adhesive, prothrombic properties of these cells. It is also involved in maintaining lysosome acidity and protecting the lysosomal membranes from auto digestion, and their expression is increased in patients with lysosomal storage disorders.The gene for LAMP1 is present on chromosome 13q34

UniProt: P11279

Pathways: Autophagie