APAF1 Antikörper (N-Term)

Produktnummer ABIN2745508

Der Ratte Monoklonal anti-APAF1 Antikörper (Klon 2E12) (ABIN2745508) detektiert spezifisch APAF1 in WB, IHC, ELISA, ICC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für APAF1 Antikörper (N-Term) (ABIN2745508)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 2E12

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 1-464, N-Term

Verwendungszweck: anti-Apaf-1 (human), mAb (2E12)

Spezifität: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Does not cross-react with mouse or rat Apaf-1.

Produktmerkmale: Monoclonal Antibody. Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 2.00E+12. Applications: ELISA, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf-1

Hintergrund:

Alternate Names/Synonyms: Apoptosis Protease-activating Factor 1

Product Description: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity