CD3 epsilon Antikörper

Produktnummer ABIN2739340

Dieser Anti-CD3 epsilon-Antikörper ist ein Hamster-Monoklonal-Antikörper zum Nachweis von CD3 epsilon in FACS, WB, IP und ELISA. Geeignet für Maus.

Kurzübersicht für CD3 epsilon Antikörper (ABIN2739340)

Target: CD3 epsilon (CD3E)

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Klon: 145-2C11

Produktdetails anti-CD3 epsilon Antikörper

Spezifität: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Aufreinigung: Protein G purified

Immunogen: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD3 antibody diluted to 10 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33 % sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, avoid freeze-thaw cycles.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gen-ID: 916

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway