CD3 epsilon Antikörper (FITC)

Produktnummer ABIN2739339

Der Hamster Monoklonal Anti-CD3 epsilon-Antikörper wurde für FACS, WB, IP und ELISA validiert. Er ist geeignet, CD3 epsilon in Proben von Maus zu detektieren.

Kurzübersicht für CD3 epsilon Antikörper (FITC) (ABIN2739339)

Target: CD3 epsilon (CD3E)

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Klon: 145-2C11

Produktdetails anti-CD3 epsilon Antikörper (FITC)

Spezifität: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Aufreinigung: Protein G purified

Immunogen: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/FITC at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gen-ID: 916

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway