PD-L1 Antikörper (PE)
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- Target Alle PD-L1 Antikörper anzeigen
- PD-L1 (CD274 (PD-L1))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser PD-L1 Antikörper ist konjugiert mit PE
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Applikation
- Flow Cytometry (FACS), ELISA
- Spezifität
- Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
- Aufreinigung
- Protein A purified
- Immunogen
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Recombinant human CD274(B7-H1).
Type of Immunogen: Recombinant protein - Klon
- ANC6H1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product PD-L1 Primärantikörper
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- Applikationshinweise
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Approved: ELISA, Flo
Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. 5 x 105 per tube were washed and pre incubated 5 minutes with 20^l of 250^g/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-human CD274 at a dilution factor of 1:50 (20 ^g/mL). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not freeze.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 4°C. Do not freeze. Protect from light.
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- Target
- PD-L1 (CD274 (PD-L1))
- Andere Bezeichnung
- B7-H1 / PD-L1 / CD274 (PD-L1 Produkte)
- Synonyme
- B7-H antikoerper, B7H1 antikoerper, PD-L1 antikoerper, PDCD1L1 antikoerper, PDCD1LG1 antikoerper, PDL1 antikoerper, A530045L16Rik antikoerper, B7h1 antikoerper, Pdcd1l1 antikoerper, Pdcd1lg1 antikoerper, Pdl1 antikoerper, RGD1566211 antikoerper, CD274 molecule antikoerper, CD274 antigen antikoerper, programmed cell death 1 ligand 2 antikoerper, CD274 antikoerper, Cd274 antikoerper, PDCD1LG2 antikoerper
- Hintergrund
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Name/Gene ID: CD274
Synonyms: CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 Molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1 - Gen-ID
- 29126
- Pathways
- Cancer Immune Checkpoints
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