MYOD1 Antikörper

Produktnummer ABIN272324

Produktdetails anti-MYOD1 Antikörper

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Aufreinigung: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Klon: 5-2F

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: 1 μg/mL. Immunofluorescence. Electron microscopy. Immunoprecipitation: 2 μg/mg of protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preserative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MYOD1 (MYOD1 Produkte)

Synonyme: MYF3 antikoerper, MYOD antikoerper, PUM antikoerper, bHLHc1 antikoerper, MyoD antikoerper, XMyoD antikoerper, XtmyoD antikoerper, mf25 antikoerper, myf3 antikoerper, pum antikoerper, MUF3 antikoerper, AI503393 antikoerper, Myod-1 antikoerper, etID309723.25 antikoerper, myod antikoerper, wu:fb57a01 antikoerper, zgc:136744 antikoerper, MYOD1 antikoerper, myod1 antikoerper, mf1 antikoerper, myod1-a antikoerper, myod1-b antikoerper, myogenic differentiation 1 antikoerper, MYOD protein antikoerper, myogenic factor MyoD1 antikoerper, myogenic differentiation 1 S homeolog antikoerper, myogenic differentiation 1 L homeolog antikoerper, MYOD1 antikoerper, myod1 antikoerper, Myod1 antikoerper, myod antikoerper, myod1.S antikoerper, myod1.L antikoerper

Hintergrund: Recognizes a phosphoprotein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.Synonyms: BHLHC1, Class C basic helix-loop-helix protein 1, MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3

Gen-ID: 10090

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development