Nibrin Antikörper

Produktnummer ABIN272298

Dieses Anti-Nibrin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Nibrin in WB und EIA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Nibrin Antikörper (ABIN272298)

Target: Nibrin (NBN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nibrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Nibrin Antikörper

Spezifität: This antibody detects endogenous levels of Nibrin protein. (region surrounding Pro461)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu Nibrin

Target: Nibrin (NBN)

Andere Bezeichnung: Nibrin

Hintergrund: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and Nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95

Molekulargewicht: approx. 95 kDa

Gen-ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response