YWHAE Antikörper

Produktnummer ABIN272265

Dieses Anti-YWHAE-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von YWHAE in WB, IF, IHC (p) und EIA. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für YWHAE Antikörper (ABIN272265)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YWHAE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-YWHAE Antikörper

Spezifität: This antibody detects endogenous levels of 14-3-3 ε protein. (region surrounding Asp238)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8° for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-YWHAE Antikörper (ABIN272265)

Hu, Sun, Li, Wang, Cai, Li, Zhao: "In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122643, (2016) (PubMed).

Nagappan, Park, Park, Hong, Yumnam, Lee, Kim, Kim, Lee, Lee, Cho, Lee, Won, Cho, Kim: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." in: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, (2013) (PubMed).

Details zu YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Andere Bezeichnung: 14-3-3 Protein epsilon

Hintergrund: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: 14-3-3E, YWHAE

Molekulargewicht: approx. 29 kDa

Gen-ID: 7531

NCBI Accession: NP_006752

UniProt: P62258

Pathways: Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, M Phase