PFKFB1 Antikörper

Produktnummer ABIN272241

Der Kaninchen Polyklonal anti-PFKFB1 Antikörper (ABIN272241) detektiert spezifisch PFKFB1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PFKFB1 Antikörper (ABIN272241)

Target: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFKFB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PFKFB1 Antikörper

Spezifität: This antibody detects endogenous levels of PFKFB1/4 protein. (region surrounding Glu349)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu PFKFB1

Target: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Andere Bezeichnung: PFKFB1

Hintergrund: PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) is a bifunctional enzyme, having both kinase and phosphatase activities residing on the same enzyme subunit but having distinct active sites. PFKFB regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis, phosphofructokinase. To date, four PFKFB isozymes (PFKFB 1-4) have been described, which show differences in their tissue distribution and kinetic properties in response to allosteric effectors and hormonal signals. Among the PFKFB's PFKFB3 has the highest kinase:phosphatase ratio, in part because it lacks the characteristic serine phosphorylation site near the N-terminal that down-modulates kinase activity. PFKFB3 was first described in the rapidly growing placenta. The glucolitic rate in placenta is accelerated by anoxia and by maternal diabetes. Cancer cells maintain a high glycolytic rate even in the presence of oxygen, a phenomenon known as the Warburg effect. The glycolytic rate in the placenta, another fast-growing tissue, is accelerated by anoxia and by maternal diabetes.Synonyms: 6-biphosphatase 1, 6-phosphofructo-2-kinase/fructose-2, F6PK, PFK-2 liver, PFK/FBPase 1, PFRX

Molekulargewicht: approx. 54 kDa

Gen-ID: 5207

NCBI Accession: NP_002616

UniProt: P16118

Pathways: Regulation of Carbohydrate Metabolic Process