NCBP1 Antikörper

Produktnummer ABIN272218

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NCBP1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für NCBP1 Antikörper (ABIN272218)

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NCBP1 Antikörper

Spezifität: This antibody detects endogenous levels of CBP80 protein. (region surrounding Arg5)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu NCBP1

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Andere Bezeichnung: NCBP1

Hintergrund: In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay(NMD), which eliminates mRNAs, which prematurely terminate translation.CBP80-bound mRNA undergoes a Synonyms: CBP80, NCBP, Nuclear cap-binding protein subunit 1

Molekulargewicht: approx. 88 kDa

Gen-ID: 4686

NCBI Accession: NP_002477

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process