HNF1A Antikörper

Produktnummer ABIN272184

Der Kaninchen Polyklonal anti-HNF1A Antikörper (ABIN272184) detektiert spezifisch HNF1A in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für HNF1A Antikörper (ABIN272184)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNF1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HNF1A Antikörper

Spezifität: This antibody detects endogenous levels of TCF-1 protein. (region surrounding Phe26)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Andere Bezeichnung: HNF1 alpha / TCF1

Hintergrund: T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic β-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β-catenin and, in turn, increases the degradation of cytosolic β-catenin and inhibits the transcriptional activity of the TCF proteins. Mutations in the APC gene, which are commonly observed in colorectal carcinomas, disrupt this regulatory pathway and correlate with an accumulation of β-catenin and the increased activation of the TCF target genes.Synonyms: HNF1A, Hepatocyte nuclear factor 1-alpha, LF-B1, LFB1, Liver-specific transcription factor LF-B1, TCF-1, Transcription factor 1

Molekulargewicht: approx. 42 kDa

Gen-ID: 6927

NCBI Accession: NP_000536

UniProt: P20823

Pathways: Hormone Transport, Carbohydrate Homeostasis