GRK5 Antikörper

Produktnummer ABIN272181

Der Kaninchen Polyklonal anti-GRK5 Antikörper (ABIN272181) detektiert spezifisch GRK5 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GRK5 Antikörper (ABIN272181)

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRK5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRK5 Antikörper

Spezifität: This antibody detects endogenous levels of GRK5 protein. (region surrouding Gln382)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu GRK5

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Andere Bezeichnung: GPRK5

Hintergrund: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1, two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.Synonyms: G protein-coupled receptor kinase 5, GRK5

Molekulargewicht: approx. 68 kDa

Gen-ID: 2869

NCBI Accession: NP_005299

UniProt: P34947

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling