HARS1/Jo-1 Antikörper

Produktnummer ABIN272164

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HARS1/Jo-1 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für HARS1/Jo-1 Antikörper (ABIN272164)

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HARS1/Jo-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HARS1/Jo-1 Antikörper

Spezifität: This antibody detects endogenous levels of HisRS protein. (region surrounding Glu492)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HARS1/Jo-1

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Andere Bezeichnung: Histidyl-tRNA Synthetase / HARS

Hintergrund: Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud's phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).Synonyms: HRS, HisRS, Histidine-tRNA ligase, JO1, Jo-1 antigen

Molekulargewicht: approx. 60 kDa

Gen-ID: 3035

NCBI Accession: NP_002100

UniProt: P12081