HARS1/Jo-1 Antikörper

Produktnummer ABIN933100

Dieser Maus Monoklonal Antikörper detektiert spezifisch HARS1/Jo-1 in WB, IP, ICC, FACS und DB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für HARS1/Jo-1 Antikörper (ABIN933100)

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HARS1/Jo-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Dot Blot (DB)

Klon: HARSA6

Produktdetails anti-HARS1/Jo-1 Antikörper

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Other species not studied.

Aufreinigung: Protein G affinity chromatography

Immunogen: HARS antibody was raised in mouse using recombinant Histidyl-Trna Synthetase

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: HARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Details zu HARS1/Jo-1

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Andere Bezeichnung: HARS

Hintergrund: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Synonyms: Monoclonal HARS antibody, Anti-HARS antibody, Histidyl-tRNA synthetase, FLJ20491 antibody, HRS antibody.