UBR5 Antikörper

Produktnummer ABIN271946

Der Kaninchen Polyklonal anti-UBR5 Antikörper wird verwendet zum Nachweis von UBR5 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für UBR5 Antikörper (ABIN271946)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-UBR5 Antikörper

Spezifität: This antibody detects endogenous levels of EDD protein. (region surrounding Thr14)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Andere Bezeichnung: UBR5 / HYD

Hintergrund: EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth (2). EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.Synonyms: E3 ubiquitin-protein ligase, E3 ubiquitin-protein ligase UBR5, EDD, EDD1, HECT domain-containing 1, Hyperplastic discs protein homolog, KIAA0896

Molekulargewicht: approx. 309 kDa

Gen-ID: 51366

NCBI Accession: NP_056986

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway