PLA2G4B Antikörper

Produktnummer ABIN271921

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PLA2G4B in WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PLA2G4B Antikörper (ABIN271921)

Target: PLA2G4B (Phospholipase A2, Group IVB (Cytosolic) (PLA2G4B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLA2G4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PLA2G4B Antikörper

Spezifität: This antibody detects endogenous levels of c-PLA2 protein. (region surrounding asn499)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: (1/10000approx. 1/20000). Western Blot (1/500approx. 1/1000). Immunohistochemistry (1/50approx. 1/200). Immunofluorescence (1/50approx. 1/200)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PLA2G4B

Target: PLA2G4B (Phospholipase A2, Group IVB (Cytosolic) (PLA2G4B))

Andere Bezeichnung: PLA2G4B

Hintergrund: Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts, and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.Synonyms: Cytosolic phospholipase A2 beta, Phospholipase A2 group IVB, cPLA2-beta, phospholipase A2 beta

Molekulargewicht: approx. 100 kDa

Gen-ID: 100137049

NCBI Accession: NP_001108105

UniProt: P0C869

Pathways: ER-Nucleus Signaling, VEGF Signaling