Ephrin B1 Antikörper

Produktnummer ABIN271886

Der Kaninchen Polyklonal anti-Ephrin B1 Antikörper wird verwendet zum Nachweis von Ephrin B1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Ephrin B1 Antikörper (ABIN271886)

Target: Ephrin B1 (EFNB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ephrin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Ephrin B1 Antikörper

Spezifität: This antibody detects endogenous levels of Ephrin-B protein. (region surrounding Gln324)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: Ephrin-B2

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ephrin B1

Target: Ephrin B1 (EFNB1)

Andere Bezeichnung: Ephrin-B1

Hintergrund: Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2

Molekulargewicht: approx. 59 kDa

Gen-ID: 1947

NCBI Accession: NP_004420

UniProt: P98172

Pathways: RTK Signalweg