SIRPA Antikörper

Produktnummer ABIN271815

Der Kaninchen Polyklonal anti-SIRPA Antikörper (ABIN271815) detektiert spezifisch SIRPA in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für SIRPA Antikörper (ABIN271815)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SIRPA Antikörper

Spezifität: This antibody detects endogenous levels of Sirp alpha1 protein. (region surrounding Arg479)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid Repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Andere Bezeichnung: CD172a / SIRPA

Hintergrund: SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-α1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-α1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-α1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-α1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-β1 shares extensive sequence homology with SIRP-α1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-γ, originally designated SIRP-β2 (SIRP-B2, CD172g) has unique characteristics from both the α and β versions. SIRP-γ is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-γ, and this interaction signals unidirectionally only.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Molekulargewicht: approx. 55 kDa

Gen-ID: 140885

NCBI Accession: NP_001035111

UniProt: P78324