CD235a/GYPA Antikörper

Produktnummer ABIN7687635

Der Maus Monoklonal anti-CD235a/GYPA Antikörper (Klon HIR2) (ABIN7687635) detektiert spezifisch CD235a/GYPA in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD235a/GYPA Antikörper (ABIN7687635)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD235a/GYPA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HIR2

Produktdetails anti-CD235a/GYPA Antikörper

Verwendungszweck: Mouse monoclonal antibody to CD235

Spezifität: Recognizes human CD235

Produktmerkmale: Mouse monoclonal antibody to CD235a

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD235a.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Andere Bezeichnung: CD235a

Hintergrund: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD antigen CD235a

Gen-ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location