Interferon gamma Antikörper

Produktnummer ABIN967468

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Interferon gamma in WB, FACS und ELISA (Detection). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Interferon gamma Antikörper (ABIN967468)

Target: Interferon gamma (IFNG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA (Detection)

Klon: 4S-B3

Produktdetails anti-Interferon gamma Antikörper

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Partially purified human IFN-gamma from supernatants of human PBMC stimulated with Staphylococcus aureus

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Blocking Control for Intracellular Staining:
The purified 4S.B3 antibody (ABIN967468) can be used as a blocking control to demonstrate specificity of IFN-gamma staining by directly conjugated clone 4S.B3. To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1-10 µg of purified 4S.B3 antibody (ABIN967468) for 20 minutes at 4°C, prior to staining with the directly conjugated antibody (e.g., 0.1 -0.5 µg mAb/1 million cells) (right panel). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

ELISA Detection:
The biotinylated 4S.B3 antibody is useful as a detection antibody in a sandwich ELISA for measuring human IFN-gamma protein levels. Biotinylated 4S.B3 antibody can be paired with the purified NIB42 antibody as the capture antibody, with recombinant human IFN-gamma as the standard. For testing human IFN-gamma complex in biological fluids like serum or plasma, our human IFN-gamma specific OptEIA™ sandwich ELISA set and OptEIA™ sandwich ELISA kit are recommended.

Western Blot:
The 4S.B3 antibody has been found useful for Western blotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml

Buffer: Aqueous buffered solution.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFN-gamma

Hintergrund: The 4S.B3 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate this hybridoma was partially purified human IFN-gamma obtained from supernatants of human PBMC stimulated with Staphylococcus aureus. This is a neutralizing antibody.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie