Cytochrome C Antikörper

Produktnummer ABIN2689486

Dieses Anti-Cytochrome C-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytochrome C in WB, IP und BI. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für Cytochrome C Antikörper (ABIN2689486)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), BioImaging (BI)

Klon: 6H2-B4

Produktdetails anti-Cytochrome C Antikörper

Marke: BD Pharmingen™

Produktmerkmale: A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c and d) according to spectrochemical characteristics, and there are five cytochromes between coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble protein that either promotes cell survival or death, depending upon its intracellular location. In healthy cells, it is a peripheral membrane protein of the mitochondria that transports electrons from the coenzyme QH2 cytochrome c reductase complex to the cytochrome c oxidase complex. When proapoptotic stimuli induce breakdown of the mitochondria, cytochrome c is released to the cytosol where it functions in the activation of caspases that trigger apoptosis. The 6H2.B4 monoclonal antibody has been reported to recognize the native and not the denatured form of rat, mouse, and human cytochrome c. Furthermore, studies utilizing competitive ELISA indicate that mAb 6H2.B4 binds to a region around residue 62 of rat cytochrome c. The 6H2.B4 monoclonal antibody is not useful for western blot analysis. For the western blot application, clone 7H8.2C12 (cat.no. 556433) would be recommended. Suggested positive controls for detecting cytochrome c include P388D mouse lymphoma cells (ATCC CCL-46), HeLa human carcinoma cells (ATCC CCL-2), Jurkat T leukemia cells (ATCC TIB-152) and NIH 3T3 mouse fibroblast cells (ATCC CRL-1658). This antibody is routinely tested by immunoprecipitation. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Cytochrome c - Purified - Clone 6H2.B4 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Cytochrome c

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Confirmed by immunoprecipitation: Human Confirmed during development by immunoprecipitation: Mouse, Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Cytochrome C Antikörper (ABIN2689486)

Garrido, Galluzzi, Brunet, Puig, Didelot, Kroemer: "Mechanisms of cytochrome c release from mitochondria." in: Cell death and differentiation, Vol. 13, Issue 9, pp. 1423-33, (2006) (PubMed).

Rossé, Olivier, Monney, Rager, Conus, Fellay, Jansen, Borner: "Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c." in: Nature, Vol. 391, Issue 6666, pp. 496-9, (1998) (PubMed).

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, (1996) (PubMed).

Jemmerson, Johnson: "Different functional boundaries for the major antigenic region of two cytochromes c." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4428-32, (1991) (PubMed).

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome C

Molekulargewicht: 15 kDa

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity