FAS Antikörper (Biotin)

Produktnummer ABIN2689462

Zitiert in 3+ Publikationen. Der Biotin-konjugierte Maus Monoklonal anti-FAS Antikörper (Klon DX2) (ABIN2689462) detektiert spezifisch FAS in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Schwein, Rhesusaffen, Hund, Pavian und Cynomolgus.

Kurzübersicht für FAS Antikörper (Biotin) (ABIN2689462)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reaktivität: Human, Schwein, Rhesusaffen, Hund, Pavian, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAS Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: DX2

Produktdetails anti-FAS Antikörper (Biotin)

Produktmerkmale: Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD95 - Biotin - Clone DX2 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Dog, Pig, Rhe - 100 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-FAS Antikörper (Biotin) (ABIN2689462)

Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, (1994) (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Details zu FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: CD95

Hintergrund: Synonyms: Fas/APO-1

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity