FAS Antikörper

Produktnummer ABIN2689461

Der Armenischer Hamster Monoklonal Anti-FAS-Antikörper wurde für FACS, IP und CyTox validiert. Er ist geeignet, FAS in Proben von Maus zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für FAS Antikörper (ABIN2689461)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser FAS Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Klon: Jo2

Produktdetails anti-FAS Antikörper

Marke: BD Pharmingen™

Produktmerkmale: Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas on mouse thymocytes analyzed by flow cytometry. Thymocytes from a C57BL/6 mouse were incubated with either Jo2, followed by a FITC-conjugated mouse anti-hamster second step (filled histogram, or with second step alone (open histogram).

BD Pharmingen™ Purified Hamster Anti-Mouse CD95 - Purified - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: WR19L mouse lymphoma cells transformed with recombinant mouse Fas

Isotyp: IgG2 lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FAS Antikörper (ABIN2689461)

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." in: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." in: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." in: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

Details zu FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: CD95

Hintergrund: Synonyms: Fas/APO-1

Molekulargewicht: 45 kDaTarget Molecular Weight: Aqueous buffered solution containing ≤0.09% sodium azide.

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity