CD86 Antikörper (Biotin)

Produktnummer ABIN2689422

Der Maus Monoklonal Anti-CD86-Antikörper wurde für FACS validiert. Er ist geeignet, CD86 in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD86 Antikörper (Biotin) (ABIN2689422)

Target: CD86

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: IT2-2

Produktdetails anti-CD86 Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: IT2.2 recognizes a 75 kDa cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes and activated B cells including lymphoid cells in germinal centers and Epstein-Barr virus transformed B-cell lines. CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in costimulation of T cells in primary immune response. Competitive binding assays demonstrate that, while both IT2.2 and FUN-1 (anti-CD86, Cat. No. 555665) recognize the same molecule, they react with different epitopes. IT2.2 blocks costimulation activity of CD86 in functional studies and blocks binding of human CTLA-4-Ig fusion protein to B70 gene-transfected cells. Profile of Daudi cells analyzed on a BD FACScan™ flowcytometer system .

BD Pharmingen™ Biotin Mouse Anti-Human CD86 - Biotin - Clone IT2.2 - Isotype Mouse IgG2b, κ - Reactivity Hu - 100 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD86 Antikörper (Biotin) (ABIN2689422)

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

Azuma, Ito, Yagita, Okumura, Phillips, Lanier, Somoza: "B70 antigen is a second ligand for CTLA-4 and CD28." in: Nature, Vol. 366, Issue 6450, pp. 76-9, (1993) (PubMed).

Nozawa, Wachi, Tominaga, Abe, Wakasa: "A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells." in: The Journal of pathology, Vol. 169, Issue 3, pp. 309-15, (1993) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund: Synonyms: B70/B7-2

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation