CD80 Antikörper (Biotin)

Produktnummer ABIN2689413

Der Armenischer Hamster Monoklonal Anti-CD80-Antikörper wurde für FACS validiert. Er ist geeignet, CD80 in Proben von Maus und Hund zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für CD80 Antikörper (Biotin) (ABIN2689413)

Target: CD80

Reaktivität: Maus, Hund

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD80 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: 16

Produktdetails anti-CD80 Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: The 16-10A1 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN-γ-activated alveolar macrophages of the dog. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of membrane CD80 by mouse splenocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141) and stained with biotinylated hamster anti-mouse CD80 clone 16-10A1 mAb (open histograms) followed by PE streptavidin (Cat. No. 554061, open and shaded histograms). Flow cytometry was performed on a FACScan™ .

BD Pharmingen™ Biotin Hamster Anti-Mouse CD80 - Biotin - Clone 16-10A1 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms, Dog - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CD80 (B7) Transfected Cell Line

Isotyp: IgG2 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD80 Antikörper (Biotin) (ABIN2689413)

Sojka, Donepudi, Bluestone, Mokyr: "Melphalan and other anticancer modalities up-regulate B7-1 gene expression in tumor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6230-6, (2000) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Boussiotis, Gribben, Freeman, Nadler: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, (1995) (PubMed).

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, (1992) (PubMed).

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund: Synonyms: B7-1

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints