CD80 Antikörper (Biotin)
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- Target Alle CD80 Antikörper anzeigen
- CD80
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Reaktivität
- Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD80 Antikörper ist konjugiert mit Biotin
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Applikation
- Flow Cytometry (FACS)
- Marke
- BD Pharmingen™
- Produktmerkmale
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The 3H5 antibody reacts with CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates. CD80 expression on spleen cells. 72-hour LPS-stimulated LOU splenocytes were simultaneously stained with FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and biotinylated mAb 3H5, followed by Streptavidin-PE (Cat. no. 554061). Viable lymphocyte blasts are represented. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Rat CD80 - Biotin - Clone 3H5 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Rat HTLV-1-transformed T-cell line Lewis-S1
- Klon
- 3H5
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD80 Primärantikörper
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
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Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
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- Target
- CD80
- Andere Bezeichnung
- CD80 (CD80 Produkte)
- Synonyme
- B7 antikoerper, B7-1 antikoerper, B7.1 antikoerper, BB1 antikoerper, CD28LG antikoerper, CD28LG1 antikoerper, LAB7 antikoerper, B71 antikoerper, Cd28l antikoerper, Ly-53 antikoerper, Ly53 antikoerper, MIC17 antikoerper, TSA1 antikoerper, CD80 molecule antikoerper, Cd80 molecule antikoerper, CD80 antigen antikoerper, CD80 antikoerper, Cd80 antikoerper
- Hintergrund
- Synonyms: B7-1
- Pathways
- T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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