CD73 Antikörper

Produktnummer ABIN2689392

Der Maus Monoklonal Anti-CD73-Antikörper wurde für FACS, IF, IP, IHC (fro), IHC (f) und IHC (zinc) validiert. Er ist geeignet, CD73 in Proben von Ratte zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für CD73 Antikörper (ABIN2689392)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD73 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Klon: 5F-B9

Produktdetails anti-CD73 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The 5F/B9 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 68- kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart. The expression of CD73 on mouse leukocytes has been well characterized. In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes, NK cells, and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes. The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT. Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18 (Cat. no. 554829). Two-color analysis of the expression of CD73 on rat spleen leukocytes. LEW splenocytes were stained with FITC- conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and either purified mouse IgG1, κ isotype control mAb MOPC-31C Cat. no. 557273, Left panel) or purified mAb 5F/B9 (Right panel), followed by biotinylated anti-mouse IgG1 mAb A85-1 (Cat. no. 553441), then Streptavidin-APC (Cat. no. 554067). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD73 - Purified - Clone 5F/B9 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Ecto-5'-nucleotidase purified from rat renal membranes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD73 Antikörper (ABIN2689392)

Yamashita, Hooker, Jiang, Laurent, Resta, Khare, Coe, Kincade, Thompson: "CD73 expression and fyn-dependent signaling on murine lymphocytes." in: European journal of immunology, Vol. 28, Issue 10, pp. 2981-90, (1998) (PubMed).

Mlodzik, Loffing, Le Hir, Kaissling: "Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart." in: Histochemistry and cell biology, Vol. 103, Issue 3, pp. 227-36, (1995) (PubMed).

Zenker, Rinne, Bankoul, Le Hir, Kaissling: "5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study." in: Histochemistry, Vol. 98, Issue 2, pp. 135-9, (1992) (PubMed).

Zimmermann: "5'-Nucleotidase: molecular structure and functional aspects." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 345-65, (1992) (PubMed).

Gandhi, Le Hir, Kaissling: "Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody." in: Histochemistry, Vol. 95, Issue 2, pp. 165-74, (1991) (PubMed).

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: CD73

Hintergrund: Synonyms: Ecto-5'-nucleotidase

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process