CD4 Antikörper

Produktnummer ABIN2689230

Der Ratte Monoklonal Anti-CD4-Antikörper wurde für FACS, IHC (fro), IHC (f) und IHC (zinc) validiert. Er ist geeignet, CD4 in Proben von Maus zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für CD4 Antikörper (ABIN2689230)

Target: CD4

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Klon: RM4

Produktdetails anti-CD4 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The RM4-5 monoclonal antibody specifically binds to the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells. CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone. Immunohistochemical staining of mouse CD4. Frozen sections of normal mouse spleen were stained with the anti-mouse CD4 antibody clone RM4-5. CD4+ T lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone RM4-5 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Thymocytes (BALB/c)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 62.5 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD4 Antikörper (ABIN2689230)

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, (1991) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: Synonyms: Cd4, CD4 antigen, L3T4, Ly-4, T-cell surface antigen T4/Leu-3

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events