FZD4 Antikörper

Produktnummer ABIN2689205

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FZD4 in WB, FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 8+ Publikationen erwähnt.

Kurzübersicht für FZD4 Antikörper (ABIN2689205)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FZD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: CH3A4A7

Produktdetails anti-FZD4 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The CH3A4A7 monoclonal antibody reacts with an extracellular epitope on human CD344 which is also known as Frizzled family receptor 4 (FZD4) or Frizzled-4. CD344 is a member of the Frizzled family of proteins within the G protein-coupled receptor (GPCR) superfamily. CD344 is a receptor for Wnt proteins and participates in the activation of the Wnt/beta-catenin pathway (canonical Wnt signaling). Patients with familial exudative vitreoretinopathy (FEVR) carry mutations in Frizzled-4. This disease is characterized by retinal degeneration and hearing loss. On tissues, CD344 is almost ubiquitously expressed. CD344 is highly expressed in adult heart and skeletal muscle. Flow Cytometric and Immunohistochemical Analysis of CD344 Expression. Panel A: Flow cytometric analysis of CD344 expression on human cell lines. Cells from the Weri-RB-1 Retinoblastoma Cell Line (ATCC HTB-169, solid line histogram) and Daudi Burkitt's Lymphoma Cell Line (ATCC CCL-213, dashed line histogram) were stained with Purified Mouse Anti-Human CD344 antibody (Cat. No. 563657). The cells were then washed and stained with PE Polyclonal Goat Anti-Mouse Ig (Cat. No.550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Panel B: Immunohistochemical analysis of CD344 expression by cardiac muscle. Acetone-fixed, frozen human heart sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, Left Image) or Purified Mouse Anti-Human CD344 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:40x.

BD Pharmingen™ Purified Mouse Anti-Human CD344 - Purified - Clone CH3A4A7 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human neuroblastoma Cell Line

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FZD4 Antikörper (ABIN2689205)

Wirths, Malenke, Kluba, Rieger, Müller, Schleicher, Hann von Weyhern, Nagl, Fend, Vogel, Mayer, Kanz, Bühring, Kopp: "Shared cell surface marker expression in mesenchymal stem cells and adult sarcomas." in: Stem cells translational medicine, Vol. 2, Issue 1, pp. 53-60, (2013) (PubMed).

Ells, Guernsey, Wallace, Zheng, Vincer, Allen, Ingram, DaSilva, Siebert, Sheidow, Beis, Robitaille: "Severe retinopathy of prematurity associated with FZD4 mutations." in: Ophthalmic genetics, Vol. 31, Issue 1, pp. 37-43, (2010) (PubMed).

Nikopoulos, Venselaar, Collin, Riveiro-Alvarez, Boonstra, Hooymans, Mukhopadhyay, Shears, van Bers, de Wijs, van Essen, Sijmons, Tilanus, van Nouhuys, Ayuso, Hoefsloot, Cremers: "Overview of the mutation spectrum in familial exudative vitreoretinopathy and Norrie disease with identification of 21 novel variants in FZD4, LRP5, and NDP." in: Human mutation, Vol. 31, Issue 6, pp. 656-66, (2010) (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).

Gregorieff, Pinto, Begthel, Destrée, Kielman, Clevers: "Expression pattern of Wnt signaling components in the adult intestine." in: Gastroenterology, Vol. 129, Issue 2, pp. 626-38, (2005) (PubMed).

MacDonald, Goldberg, Macfarlane, Samuels, Trese, Shastry: "Genetic variants of frizzled-4 gene in familial exudative vitreoretinopathy and advanced retinopathy of prematurity." in: Clinical genetics, Vol. 67, Issue 4, pp. 363-6, (2005) (PubMed).

Xu, Wang, Dabdoub, Smallwood, Williams, Woods, Kelley, Jiang, Tasman, Zhang, Nathans: "Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair." in: Cell, Vol. 116, Issue 6, pp. 883-95, (2004) (PubMed).

Kirikoshi, Sagara, Koike, Tanaka, Sekihara, Hirai, Katoh: "Molecular cloning and characterization of human Frizzled-4 on chromosome 11q14-q21." in: Biochemical and biophysical research communications, Vol. 264, Issue 3, pp. 955-61, (1999) (PubMed).

Details zu FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Andere Bezeichnung: CD344

Hintergrund: Synonyms: FZD4, Frizzled family receptor 4, Frizzled-4, Fz-4, FzE4, hFz4, EVR1, FEVR

Pathways: WNT Signalweg, Hormone Transport, Sensory Perception of Sound