CD31 Antikörper

Produktnummer ABIN2689181

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD31 in FACS, IHC, ELISA, IHC (p), IP und BR. Es zeigt Reaktivität gegenüber Proben von Ratte und Schwein. Es wurde in 5+ Publikationen zitiert.

Kurzübersicht für CD31 Antikörper (ABIN2689181)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Blocking Reagent (BR)

Klon: TLD

Produktdetails anti-CD31 Antikörper

Produktmerkmale: The TLD-3A12 antibody reacts with CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin super family, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat. In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leukocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis. The TLD-3A12 mAb partially blocks the proliferation response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This mouse anti-rat CD31 antibody cross-reacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively. A suspension of Lewis rat microglial cells were used as the source of the immunogen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. The expression of CD31 on rat spleen leukocytes. LEW splenocytes were stained with purified mAb TLD-3A12 (right panel) followed by biotinylated anti-mouse Igg1 mAb A85-1 (Cat. No. 553441), then FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. No. 554883) and Streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD31 - Purified - Clone TLD-3A12 - Isotype Mouse IgG1, κ - Reactivity Rat, Pig - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Lewis Rat Microglia

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD31 Antikörper (ABIN2689181)

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Famiglietti, Sun, DeLisser, Albelda: "Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1425-35, (1997) (PubMed).

Williams, Zhao, Ueno, Hickey: "PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat." in: Journal of neuroscience research, Vol. 45, Issue 6, pp. 747-57, (1997) (PubMed).

Wakelin, Sanz, Dewar, Albelda, Larkin, Boughton-Smith, Williams, Nourshargh: "An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 229-39, (1996) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31

Hintergrund: Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization