SIRPA Antikörper

Produktnummer ABIN2689017

Der Ratte Monoklonal Anti-SIRPA-Antikörper wurde für WB, FACS, IP, IF und BR validiert. Er ist geeignet, SIRPA in Proben von Maus zu detektieren. Es sind 7+ Publikationen verfügbar.

Kurzübersicht für SIRPA Antikörper (ABIN2689017)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SIRPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR)

Klon: P84

Produktdetails anti-SIRPA Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The P84 antibody reacts with CD172a, also known as SIgnal-Regulatory Protein α (SIRPα), Src Homology 2 domain-containing protein tyrosine Phosphatase (SHP) Substrate 1 (SHPS-1), or Brain Immunoglobulin-like molecule with Tyrosine-based activation motifs (BIT). CD172a is an adhesion molecule of the Ig superfamily which is expressed on neurons in the central nervous system and the retina, on macrophages, and on bone-marrow myeloid cells. Its ligand, CD47, or Integrin-Associated Protein (IAP), is expressed by a wide variety of cells. CD172a and CD47 are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. Two-color analysis of CD172a expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG1 isotype control mAb (left panel) or purified mAb P84 (right panel), followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892, both panels), then PE-conjugated anti-mouse CD11b (Mac-1) mAb M1/70 (Cat. No. 557397/553311). Total viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibura .

BD Pharmingen™ Purified Rat Anti-Mouse CD172a - Purified - Clone P84 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Membrane fractions from mouse brain

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SIRPA Antikörper (ABIN2689017)

Mi, Jiang, Weng, Lindberg, Narayanan, Lagenaur: "Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina." in: The Journal of comparative neurology, Vol. 416, Issue 3, pp. 335-44, (2000) (PubMed).

Gresham, Dale, Potter, Chang, Vines, Lowell, Lagenaur, Willman: "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." in: The Journal of experimental medicine, Vol. 191, Issue 3, pp. 515-28, (2000) (PubMed).

Oldenborg, Zheleznyak, Fang, Lagenaur, Gresham, Lindberg: "Role of CD47 as a marker of self on red blood cells." in: Science (New York, N.Y.), Vol. 288, Issue 5473, pp. 2051-4, (2000) (PubMed).

Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, (1999) (PubMed).

Veillette, Thibaudeau, Latour: "High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22719-28, (1998) (PubMed).

Comu, Weng, Olinsky, Ishwad, Mi, Hempel, Watkins, Lagenaur, Narayanan: "The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 22, pp. 8702-10, (1997) (PubMed).

Chuang, Lagenaur: "Central nervous system antigen P84 can serve as a substrate for neurite outgrowth." in: Developmental biology, Vol. 137, Issue 2, pp. 219-32, (1990) (PubMed).

Details zu SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Andere Bezeichnung: CD172a

Hintergrund: Synonyms: SIRPα, SHPS-1, BIT