PDGFRA Antikörper

Produktnummer ABIN2688972

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-PDGFRA Antikörper wird verwendet zum Nachweis von PDGFRA in Proben von Human. Er wurde validiert für FACS, IP und Func.

Kurzübersicht für PDGFRA Antikörper (ABIN2688972)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Klon: AlphaR1

Produktdetails anti-PDGFRA Antikörper

Produktmerkmale: Reacts with platelet derived growth factor (PDGF) receptor α, CD140a, a 170 kD single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. They are structurally similar to M-CSF receptor and CD117 (c-kit). Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells. PDGF plays a role in wound healing and it also acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils. Functional PDGF is secreted as a dimer, disulfide linked, A and B chains (PDGFAA, PDGF-BB or PDGF-AB). Binding of divalent PDGF induces receptor dimerization with three possible forms: αα, αβ, ββ. The PDGF receptor α-subunit binds both PDGF A and B chains, whereas the receptor β-subunit binds only PDGF B chains. Both receptor subunits can stimulate mitogenic responses, only the β subunit can induce chemotaxis. αR1 is specific for αPDGFR and does not cross react with βPDGFR. It immunoprecipitates human, monkey, rabbit, pig, dog and cat αPDGFR. It does not recognize hamster, rat or mouse αPDGFR. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of MG-63 (osteosarcoma cell line) cells analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD140a - Purified - Clone αR1 (also known as Alpha-R1) - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-PDGFRA Antikörper (ABIN2688972)

Bazenet, Kazlauskas: "The PDGF receptor alpha subunit activates p21ras and triggers DNA synthesis without interacting with rasGAP." in: Oncogene, Vol. 9, Issue 2, pp. 517-25, (1994) (PubMed).

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: CD140a

Hintergrund: Synonyms: PDGF receptor α chain

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling