Syndecan 1 Antikörper (Biotin)

Produktnummer ABIN2688968

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Syndecan 1 in FACS, IF und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für Syndecan 1 Antikörper (Biotin) (ABIN2688968)

Target: Syndecan 1 (SDC1)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Syndecan 1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 281

Produktdetails anti-Syndecan 1 Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. Expression of CD138 on mouse bone- arrow B lymphocytes. C57BL/6 bone- arrow leukocytes were simultaneously stained with biotin-conjugated 281-2 mAb (Right panel) and FITC-conjugated RA3-6B2 mAb (anti- D45R/B220, Cat. no. 553087/553088, both panels), followed by Streptavidin-PE (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Biotin Mouse Anti-CD138 - Biotin - Clone 281-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: NAMRU mouse mammary gland epithelial cell line NMuMG

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry System.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-Syndecan 1 Antikörper (Biotin) (ABIN2688968)

Wehrli, Legler, Finke, Toellner, Loetscher, Baggiolini, MacLennan, Acha-Orbea: "Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes." in: European journal of immunology, Vol. 31, Issue 2, pp. 609-16, (2001) (PubMed).

Details zu Syndecan 1

Target: Syndecan 1 (SDC1)

Andere Bezeichnung: CD138

Hintergrund: Synonyms: Syndecan-1

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism