LAMP2 Antikörper

Produktnummer ABIN2688888

Der Ratte Monoklonal Anti-LAMP2-Antikörper wurde für FACS, IP, IHC (fro), IHC (p) und IHC (zinc) validiert. Er ist geeignet, LAMP2 in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für LAMP2 Antikörper (ABIN2688888)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Klon: M3-84

Produktdetails anti-LAMP2 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The M3/84 monoclonal antibody specifically binds to CD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 (LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). CD107b is a single-pass type I transmembrane glycoprotein that constitutes a major integral membrane protein of lysosomes and may play a role in lysosomal function. CD107b is also expressed on the surface of mouse mononuclear phagocytes. Surface expression of the 92-110- kDa glycoprotein antigen increases during differentiation of monocytes to activated macrophages and may play a role in adhesion. The M3/84 mAb can detect CD107b antigen on tissue macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), and gut-associated lymphoid tissue. Immunohistochemical staining of macrophages. The formalin-fixed paraffin-embedded sections of normal mouse lung were stained with M3/84 mAb (left panel) or R3-34 mAb (isotype control, right panel). Macrophages are identified by the brown labeling of their cell -surface membranes.

BD Pharmingen™ Purified Rat Anti-Mouse CD107b - Purified - Clone M3/84 - Isotype Rat IgG1, κ - Reactivity Ms - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse C57Bl/6 peritoneal exudate cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-LAMP2 Antikörper (ABIN2688888)

Walker, Akporiaye, Warner, Stewart: "Characterization of subsets of bone marrow-derived macrophages by flow cytometry analysis." in: Journal of leukocyte biology, Vol. 37, Issue 2, pp. 121-36, (1985) (PubMed).

Chen, Murphy, Willingham, Pastan, August: "Identification of two lysosomal membrane glycoproteins." in: The Journal of cell biology, Vol. 101, Issue 1, pp. 85-95, (1985) (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, (1983) (PubMed).

Ralph, Ho, Litcofsky, Springer: "Expression and induction in vitro of macrophage differentiation antigens on murine cell lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 1, pp. 108-14, (1983) (PubMed).

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: CD107b

Hintergrund: Synonyms: CD107b, Lamp2, LAMP-2, Lysosome-associated membrane glycoprotein 2, LGP-B

Pathways: Autophagie