BrdU Antikörper

Produktnummer ABIN2688858

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-BrdU Antikörper wird verwendet zum Nachweis von BrdU. Er wurde validiert für IHC (fro), FM und ICS.

Kurzübersicht für BrdU Antikörper (ABIN2688858)

Target: BrdU (Bromodeoxyuridine (BrdU))

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence Microscopy (FM), Intracellular Staining (ICS)

Klon: 3D4

Produktdetails anti-BrdU Antikörper

Produktmerkmale: Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the vells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU. Fluorescence Intensity (Linear) Profile of KG1A cells analyzed ona FACScan

BD Pharmingen™ Purified Mouse Anti- BrdU - Purified - Clone 3D4 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-BrdU Antikörper (ABIN2688858)

Favero, Henshaw, Grimsley-Myers, Shrestha, Beier, Dwyer: "Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain." in: The Journal of comparative neurology, Vol. 521, Issue 3, pp. 677-96, (2013) (PubMed).

Allison, Barthel, Skebo, Takechi, Kawamura, Raymond: "Ontogeny of cone photoreceptor mosaics in zebrafish." in: The Journal of comparative neurology, Vol. 518, Issue 20, pp. 4182-95, (2011) (PubMed).

Navailles, Hof, Schmauss: "Antidepressant drug-induced stimulation of mouse hippocampal neurogenesis is age-dependent and altered by early life stress." in: The Journal of comparative neurology, Vol. 509, Issue 4, pp. 372-81, (2008) (PubMed).

Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical